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Please use this identifier to cite or link to this item: https://hdl.handle.net/11055/1279
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dc.contributor.authorO’Brien JAen_US
dc.contributor.authorMcGuire HMen_US
dc.contributor.authorShinko Den_US
dc.contributor.authorFazekas de St Groth Ben_US
dc.contributor.authorRusso Men_US
dc.contributor.authorBailey Den_US
dc.contributor.authorSantarelli DMen_US
dc.contributor.authorWynne Ken_US
dc.contributor.authorAustin PJen_US
dc.date2021-08-
dc.date.accessioned2025-01-14T04:35:56Z-
dc.date.available2025-01-14T04:35:56Z-
dc.date.issued2021-06-09-
dc.identifier.citation15:100283.en_US
dc.identifier.issn2666-3546en_US
dc.identifier.urihttps://hdl.handle.net/11055/1279-
dc.description.abstractDiabetic neuropathic pain is a common and devastating complication of type 1 diabetes, but the mechanism by which it develops and persists is yet to be fully elucidated. This study utilised high-dimensional suspension mass cytometry in a pilot cohort to investigate differences in peripheral blood immunophenotypes between type 1 diabetes patients with (n ​= ​9) and without (n ​= ​9) peripheral neuropathic pain. The abundance and activation of several leukocyte subsets were investigated with unsupervised clustering approaches FlowSOM and SPADE, as well as by manual gating. Major findings included a proportional increase in CD4+ central memory T cells and an absolute increase in classical monocytes, non-classical monocytes, and mature natural killer cells in type 1 diabetes patients with pain compared to those without pain. The expression of CD27, CD127, and CD39 was upregulated on select T cell populations, and the phosphorylated form of pro-inflammatory transcription factor MK2 was upregulated across most populations. These results provide evidence that distinct immunological signatures are associated with painful neuropathy in type 1 diabetes patients. Further research may link these changes to mechanisms by which pain in type 1 diabetes is initiated and maintained, paving the way for much needed targeted treatments.en_US
dc.subjectCD27en_US
dc.subjectChronic painen_US
dc.subjectDiabetic neuropathyen_US
dc.subjectFlowSOMen_US
dc.subjectImmunophenotypingen_US
dc.subjectMAPKAPK2en_US
dc.subjectMK2en_US
dc.subjectMass cytometryen_US
dc.subjectSPADEen_US
dc.subjectType 1 diabetesen_US
dc.titleT lymphocyte and monocyte subsets are dysregulated in type 1 diabetes patients with peripheral neuropathic painen_US
dc.typeJournal Articleen_US
dc.type.contentTexten_US
dc.identifier.journaltitleBrain, Behavior, & Immunity – Healthen_US
dc.identifier.doi10.1016/j.bbih.2021.100283en_US
dc.description.affiliatesSchool of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Brain and Mind Centre, 94 Mallett St, Camperdown, NSW, 2050, Australia.en_US
dc.description.affiliatesDiscipline of Pathology, Faculty of Medicine and Health, The University of Sydney, NSW, Australia.en_US
dc.description.affiliatesRamaciotti Facility for Human Systems Biology, Charles Perkins Centre, The University of Sydney, NSW, Australia.en_US
dc.description.affiliatesSydney Cytometry, The University of Sydney, NSW, Australia.en_US
dc.description.affiliatesGenesis Research Services, Broadmeadow, NSW, Australia.en_US
dc.description.affiliatesHunter Medical Research Institute, New Lambton Heights, NSW, Australia.en_US
dc.description.affiliatesDepartment of Diabetes and Endocrinology, John Hunter Hospital, Newcastle, NSW, Australia.en_US
dc.description.affiliatesSchool of Medicine and Public Health, University of Newcastle, NSW, Australia.en_US
dc.description.pubmedurihttps://pubmed.ncbi.nlm.nih.gov/34589782/en_US
dc.type.studyortrialStudyen_US
dc.type.specialtyAnaesthesiaen_US
dc.type.specialtyPain Medicineen_US
dc.identifier.fulltextlinkhttps://www.sciencedirect.com/science/article/pii/S2666354621000867?via%3Dihuben_US
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextrestricted-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.openairetypeJournal Article-
Appears in Collections:Scholarly and Clinical
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